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Expression of leukotriene and its receptors in eosinophilic chronic rhinosinusitis with nasal polyps
Author(s) -
Wu Xingmei,
Hong Haiyu,
Zuo Kejun,
Han Miaomiao,
Li Jian,
Wen Weiping,
Xu Geng,
Miao Beiping,
Li Huabin
Publication year - 2016
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.21625
Subject(s) - nasal polyps , medicine , eosinophilic , leukotriene receptor , leukotriene , montelukast , messenger rna , receptor , major basic protein , immunohistochemistry , immunology , reverse transcription polymerase chain reaction , western blot , pathogenesis , antagonist , eosinophil , endocrinology , pathology , biology , asthma , gene , biochemistry
Background Cysteinyl leukotriene (LT) has been proposed in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). This study sought to examine the expression of the LT receptor (LTR) in CRSwNP patients and evaluate the potential role of LTR antagonist (LTRA) in the management of eosinophilic CRSwNP (ECRS) patients. Methods Nasal polyps and uncinate process tissues were collected from 18 ECRS patients, 13 non‐eosinophilic CRSwNP (non‐ECRS) patients, and 16 control subjects. The messenger RNA (mRNA) and protein expression of LTR (cysteinyl leukotriene receptor 1 [CysLT1R] and cysteinyl leukotriene receptor 2 [CysLT2R]) was examined using quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR), immunohistochemistry, and Western blot analysis. Moreover, the effects of LTRA and steroids on total nasal symptom scores (TNSS) of uncontrolled ECRS patients were evaluated. Results The mRNA and protein expression of CysLT1R and CysLT2R was significantly increased in polyp tissues compared with healthy controls ( p < 0.05). Compared with the non‐ECRS subset, the ECRS subset showed significantly increased expression of CysLT1R and CysLT2R, as well as leukotriene C4 (LTC4) and leukotriene D4 (LTC4) levels ( p < 0.05). Moreover, combined LTRA and steroids significantly decreased TNSS more than steroids alone in uncontrolled ECRS patients ( p < 0.01). Conclusion Our findings indicate that LTR was differentially expressed between ECRS and non‐ECRS patients, and that LTRA may be used as an additional therapy for ECRS patients.