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Interleukin‐17A–induced inflammation does not influence the development of nasal polyps in murine model
Author(s) -
Hong SungLyong,
Zhang YuLian,
Kim SangWook,
Kim Dae Woo,
Cho SangHeon,
Chang YoonSeok,
Lee Chul Hee,
Rhee ChaeSeo
Publication year - 2015
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.21515
Subject(s) - medicine , nasal polyps , inflammation , mucous membrane of nose , proinflammatory cytokine , pathology , eotaxin , sirius red , immunology , eosinophil , interleukin , ovalbumin , interleukin 17 , masson's trichrome stain , nasal cavity , immunohistochemistry , cytokine , chemokine , immune system , asthma , surgery
Background Nasal polyposis associated with chronic rhinosinusitis (CRS) is a chronic inflammatory disease that is characterized by infiltration of many inflammatory cells. Meanwhile, interleukin (IL)‐17A is a well‐known proinflammatory cytokine that induces both eosinophilic and neutrophilic inflammation. We investigated the role of IL‐17A in the development of nasal polyps in the CRS murine model. Methods Eosinophilic CRS with nasal polyps was induced by using ovalbumin (OVA) and Staphylococcus aureus enterotoxin B (SEB) in wild‐type BALB/c and IL‐17A knockout (KO) mice. Histopathologic changes of the sinonasal cavity were evaluated using hematoxylin and eosin, Periodic acid‐Schiff, Sirius red, Masson's trichrome, and immunohistochemistry. The levels of total and OVA‐specific immunoglobulin Es (IgEs) in sera were measured using enzyme‐linked immunosorbent assay. The expression levels of IL‐4, IL‐5, and interferon‐γ (IFN‐γ) in the nasal mucosa were assessed by quantitative real‐time polymerase chain reaction. Results Under the IL‐17A deficiency, total and OVA‐specific IgEs in sera were reduced significantly. Infiltration of both eosinophils and neutrophils into the nasal mucosa, subepithelial fibrosis, and goblet cell count also decreased significantly in IL‐17A KO mice treated with both OVA and SEB compared with those in the wild‐type counterpart. However, there were no significant differences in the number of polypoid lesions among groups. Meanwhile, IL‐4 increased and IFN‐γ decreased in the nasal mucosa in IL‐17A KO mice treated with both OVA and SEB. Conclusion This study suggests that even though IL‐17A plays an important role in both nasal inflammation and remodeling, it does not influence the development of nasal polypoid lesions.