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Role of platelet‐derived growth factor‐α in eosinophilic and non‐eosinophilic chronic rhinosinusitis with nasal polyps
Author(s) -
Lin Hai,
Lin Dong,
Xiong XiSheng,
Dai XiongXiong,
Lin Ting
Publication year - 2014
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.21419
Subject(s) - nasal polyps , platelet derived growth factor receptor , medicine , platelet derived growth factor , growth factor , eosinophilic , immunohistochemistry , immunostaining , immunology , messenger rna , pathogenesis , pathology , receptor , biology , gene , biochemistry
Background The pathogenesis of human chronic rhinosinusitis with nasal polyps (CRSwNP) comprising eosinophilic CRSwNP (ECRSwNP) and non‐eosinophilic (nECRSwNP) is not completely understood. Recent evidence has suggested that platelet‐derived growth factor receptor alpha (PDGFRα) is implicated in cell growth, transformation, proliferation, migration, and vascular permeability and platelet‐derived growth factor‐A (PDGF‐A) is a specific ligand for PDGFRα. However, little is known about their roles in CRSwNP. Therefore, we aimed to investigate the expression and role of PDGFRα and PDGF‐A in CRSwNP. Methods PDGFRα protein expression was investigated by immunohistochemistry method and messenger RNA (mRNA) expression of PDGFRα and PDGF‐A were assessed by real‐time polymerase chain reaction (PCR) in CRSwNP patients and control subjects. Moreover, the effects of various stimulators with different concentrations and time on PDGFRα were evaluated on nasal explant culture. Results Quantitative analysis of immunostaining for PDGFRα showed an obvious elevation in immunolabeling of PDGFRα in CRSwNP groups compared with controls. Furthermore, PDGFRα protein was significantly stronger expressed in ECRSwNP group than nECRSwNP group and atopic patients showed stronger expression of PDGFRα protein than nonatopic patients. The mRNA of PDGFRα and PDGF‐A were overexpressed in CRSwNP, especially in ECRSwNP. PDGFRα mRNA expression was closely related to PDGF‐A mRNA. In nasal explant culture and stimulation, PDGFRα mRNA was augmented by interleukin 4 (IL‐4), IL‐5, or IL‐1β respectively, but suppressed by IL‐27. Conclusion PDGFRα may play a pivotal role in the pathophysiology of ECRSwNP and nECRSwNP by combining with PDGF‐A. IL‐4, IL‐5, or IL‐1β may be critical for PDGFRα gene expression.