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Probiotic manipulation of the chronic rhinosinusitis microbiome
Author(s) -
Cleland Edward John,
Drilling Amanda,
Bassiouni Ahmed,
James Craig,
Vreugde Sarah,
Wormald PeterJohn
Publication year - 2014
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.21279
Subject(s) - probiotic , medicine , staphylococcus epidermidis , microbiome , sinusitis , nasal administration , chronic rhinosinusitis , staphylococcus aureus , saline , microbiology and biotechnology , immunology , pathogenic bacteria , goblet cell , chronic sinusitis , bacteria , gastroenterology , pathology , biology , bioinformatics , genetics , epithelium
Background Staphylococcus aureus (SA) is a key pathogenic component of the chronic rhinosinusitis (CRS) microbiome and is associated with increased disease severity and poor postoperative outcomes. Probiotic treatments potentially offer a novel approach to the management of pathogenic bacteria in these recalcitrant patients through supporting a healthy community of commensal species. This study aims to investigate the probiotic properties of Staphylococcus epidermidis (SE) against SA in a mouse model of sinusitis. Methods Twenty C57/BL6 mice received intranasal inoculations of phosphate buffered saline (PBS), SE, SA, or a combination of SE and SA (SE+SA) for 3 days. Following euthanasia, the mouse snouts were harvested and prepared for histological analysis. Counts of periodic acid–Schiff (PAS)‐positive goblet cells were the primary outcome measure. Results Goblet cell counts were significantly higher in both the SA and SE+SA groups compared to those receiving PBS or SE alone ( p < 0.05). However, the SE+SA group demonstrated significantly lower goblet cell counts compared to the SA group ( p < 0.05). Mice receiving SE alone did not show a significant difference to those receiving PBS ( p > 0.05). The presence of SA postinoculation was confirmed by culture in both the SA and SE+SA groups. Conclusion This study confirms the probiotic potential of SE against SA in a mouse model of sinusitis. Although the interactions that occur between many probiotic species and pathogens are yet to be fully understood, studies such as this support further exploration of ecologically‐based treatment paradigms for the management of CRS.