Premium
Characterization of B‐cell subpopulations in patients with chronic rhinosinusitis
Author(s) -
Psaltis Alkis J.,
Schlosser Rodney J.,
Yawn James R.,
Henriquez Oswaldo,
Mulligan Jennifer K.
Publication year - 2013
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.21173
Subject(s) - medicine , chronic rhinosinusitis , nasal polyps , flow cytometry , pathogenesis , immunology , plasma cell , b cell , sinusitis , pathology , bone marrow , antibody
Background Recent research suggest that B and plasma cells may play an important role in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The purpose of this study was to subcharacterize the B cell response in the sinus mucosa of control and CRS patients. Methods Representative tissue samples and peripheral blood samples were obtained from controls, CRS without nasal polyps (CRSsNP) and CRSwNP. Using single‐cell suspension flow cytometry these samples were analyzed for overall and stage‐specific B and plasma cell percentages. Results Both atopic and nonatopic CRSwNP patients showed an increase in local numbers of naive, active, and memory B cells compared to controls. CRSsNP patients only showed local elevations of naive B cells. Plasma cells were only significantly elevated in the sinus tissue of atopic CRSwNP patients. These local tissue increases did not correlate with increased numbers of circulating B cells. Conclusion This study provides further evidence of an important role of B cells in CRSwNP patients. The local increase appears to be independent of a systemic response.