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Technical note: Improved ancient DNA purification for PCR using ion‐exchange columns
Author(s) -
Kim Kijeong,
Kim KyungYong,
Jeon Eunhee,
Togloom Ariunaa,
Cho YounOck,
Lee MinSoo,
Lkhagvasuren Gavaachimed,
Choi JeeHye,
Tumen Dashtseveg,
Ja Park Ae,
Kim KeunCheol,
Park KiWon,
Kim JaeHyun,
Noh Maengseok,
Yoo KwonJong,
Lee KwangHo
Publication year - 2008
Publication title -
american journal of physical anthropology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.146
H-Index - 119
eISSN - 1096-8644
pISSN - 0002-9483
DOI - 10.1002/ajpa.20782
Subject(s) - ancient dna , dna extraction , dna , polymerase chain reaction , chromatography , extraction (chemistry) , ion exchange , biology , chemistry , microbiology and biotechnology , ion , gene , genetics , population , demography , organic chemistry , sociology
A novel method of ancient DNA (aDNA) purification was developed using ion‐exchange columns to improve PCR‐amplifiable DNA extraction from ancient bone samples. Thirteen PCR‐resistant ancient bone samples aged 500–3,300 years were tested to extract aDNA using a recently reported, silica‐based aDNA extraction method and an ion‐exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high‐copy DNA, and amelogenin, a low‐copy DNA. The results demonstrate that the further purification of silica‐based aDNA extracts using ion‐exchange columns considerably improved PCR amplification. We suggest that the ion‐exchange column‐based method will be useful for the improvement of PCR‐amplifiable aDNA extraction, particularly from the poorly preserved, PCR‐resistant, ancient samples. Am J Phys Anthropol, 2008. © 2008 Wiley‐Liss, Inc.