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Mitochondrial sequence variation in the Guahibo Amerindian population from Venezuela
Author(s) -
Vona Giuseppe,
Falchi Alessandra,
Moral Pedro,
Calò Carla M.,
Varesi Laurent
Publication year - 2005
Publication title -
american journal of physical anthropology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.146
H-Index - 119
eISSN - 1096-8644
pISSN - 0002-9483
DOI - 10.1002/ajpa.20070
Subject(s) - haplogroup , mitochondrial dna , hypervariable region , mtdna control region , haplotype , biology , genetics , d loop , population , polymorphism (computer science) , genetic variation , evolutionary biology , genotype , gene , demography , sociology
New data were obtained on mitochondrial DNA (mtDNA) from Guahibo from Venezuela, a group so far not studied using molecular data. A population sample (n = 59) was analyzed for mtDNA variation in two control‐region hypervariable segments (HV1 and HV2) by sequencing. The presence or absence of a 9‐bp polymorphism in the COII/tRNA Lys region was studied by direct amplification and electrophoretic identification. Thirty‐eight variable sites were detected in regions HV1 and HV2, defining 26 mtDNA lineages; 23.7% of these were present in a single individual. The 9‐bp deletion was found in 3.39% of individuals. Nucleotide and haplotype diversities were relatively high compared with other New World populations. The identified sequence haplotypes were classified into four major haplogroups (A–D) according to previous studies, with high frequencies for A (47.46%) and C (49.15%), low frequency for B (3.39%), and an absence of D. Am J Phys Anthropol, 2005. © 2005 Wiley‐Liss, Inc.