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Trypanosoma cruzi infection in captive Neotropical primates in the Brazilian Amazon
Author(s) -
Bahia Michele,
de Nazaré Leite Barros Flávia,
MagalhãesMatos Paulo Cesar,
de Souza Gonçalves Thamirys,
Chiesorin Neto Laerzio,
Oliveira Faria Diogo Cesar Lagroteria,
Aparecida Romeiro Sandra,
Barros Monteiro Frederico Ozanan,
GóesCavalcante Gustavo,
Scofield Alessandra
Publication year - 2017
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/ajp.22590
Subject(s) - trypanosoma cruzi , amazon rainforest , biology , captivity , zoology , chagas disease , virology , blood smear , amazon basin , parasite hosting , veterinary medicine , immunology , ecology , malaria , medicine , world wide web , computer science
The aim of this study was to detect the infection by Trypanosoma cruzi in captive Neotropical primates in the Brazilian Amazon. From February 2013 to July 2014, 112 blood samples were collected from Neotropical primates from the Amazonas, Amapá, and Pará States, north of Brazil. The subjects belonged to the families Cebidae ( N = 59), Atelidae ( N = 41), Callitrichidae ( N = 5), Pitheciidae ( N = 4), and Aotidae ( N = 3). Blood smears also were examined for the presence of trypomastigotes by optical microscopy. For the detection of T. cruzi DNA, a Nested‐PCR with primers TCZ1/TCZ2 and TCZ3/TCZ4 was performed. T. cruzi DNA was detected in 12.5% (14/112) of Neotropical primates examined. Positive samples were detected in 16%, 12.5%, and 11.11% of the different species of primates sampled from the Amapá, Pará, and Amazonas states, respectively. The analysis of the blood smears did not reveal trypomastigote forms of T. cruzi . In conclusion, Neotropical primates kept in captivity were infected by T. cruzi in the studied areas. We recommend that a health management protocol be put into place to prevent the transmission of infectious agents among captive populations, captive and wild populations, and between NHPs and the technicians who handle these animals.