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Polymorphic microsatellite loci for the common marmoset ( Callithrix jacchus ) designed using a cost‐ and time‐efficient method
Author(s) -
Raveendran M.,
Tardif S.,
Ross C.N.,
Austad S.N.,
Harris R.A.,
Milosavljevic A.,
Rogers J.
Publication year - 2008
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/ajp.20581
Subject(s) - marmoset , callithrix , microsatellite , biology , genetics , zoology , evolutionary biology , ecology , allele , gene
We describe a cost‐ and time‐efficient method for designing new microsatellite markers in any species with substantial genomic DNA sequence data available. Using this technique, we report 14 new polymorphic dinucleotide microsatellite loci isolated from the common marmoset. The relative yield of new polymorphisms was higher with less labor than described in previous marmoset studies. Of 20 loci initially evaluated, 14 were polymorphic and amplified reliably (70% success rate). The number of alleles ranged from 3 to 9 with heterozygosity varying from 0.48 to 0.83. Am. J. Primatol. 70:906–910, 2008. © 2008 Wiley‐Liss, Inc.