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Capacitation and the acrosome reaction in squirrel monkey ( Saimiri sciureus ) spermatozoa evaluated by the chlortetracycline fluorescence assay
Author(s) -
Kholkute S. D.,
Lian Ye,
Roudebush W. E.,
Dukelow W. R.
Publication year - 1990
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/ajp.1350200206
Subject(s) - capacitation , acrosome reaction , sperm , squirrel monkey , andrology , acrosome , biology , saimiri sciureus , staining , ionophore , semen , chemistry , endocrinology , anatomy , biochemistry , medicine , genetics , membrane
Capacitation and the acrosome reaction in squirrel monkey seminal spermatozoa diluted in Tyrode's medium (TALP) and TC‐199 were monitored by a chlortetracycline (CTC) fluorescence assay. Four CTC patterns, similar to those found in human sperm, were readily characterized by fluorescent staining on the heads of the spermatozoa. The appearance of the capacitated (CP) pattern was dependent on the concentration of the bovine serum albumin. Acrosomal loss was observed in a maximum of 15% of the sperm in the populations studied here. Calcium ionophore A23187 (5 μM to 20μM) induced acrosomal loss in 60–70% of capacitated spermatozoa. However in freshly ejaculated sperm incubated under capacitating conditions or in spermatozoa incubated in Ca + + ‐free medium, A23187 failed to induce acrosomal loss. Furthermore, spermatozoa incubated in the presence of seminal plasma or spermatozoa obtained following a 1‐hour “swim‐up” procedure showed an identical timecourse of appearance of the CP pattern, indicating the lack of effect of seminal plasma on capacitation in the squirrel monkey.