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Atresia of the dominant ovarian follicle in rhesus monkeys is detected within 24 hours of estradiol treatment
Author(s) -
Hutz Reinhold J.,
Krueger Gail S.,
Morgan Patricia M.,
Dierschke Donald J.,
Wolf Richard C.
Publication year - 1989
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/ajp.1350180307
Subject(s) - atresia , medicine , endocrinology , follicle , estrogen , follicular phase , ovary , follicular atresia , follicular fluid , menstrual cycle , oocyte , ovarian follicle , chemistry , ovarian tissue , biology , hormone , embryo , microbiology and biotechnology
Following treatment with estradiol‐17β (E 2 ) on day 6 of the menstrual cycle, degenerative alterations in the microenvironment of the dominant follicle (DF) (follicular fluid [FF], granulosa cells [GC], and oocyte) are readily apparent on day 10, or 96 h after E 2 administration. The present study was designed to determine how early such changes could be detected and which indices of atresia were observed first. The DF was identified during laparoscopy on day 5 or 6 of the cycle, and four capsules containing crystalline E 2 were inserted s.c. for 24 h. Contents of the DF were aspirated at 24, 48, and 72 h following initiation of E 2 treatment. General size and appearance of the DF did not change distinctly with E 2 treatment; however, by 48 h FF viscosity was increased markedly. GC viability was not altered with treatment. FF concentrations of estrogen (E) were dramatically reduced at 24 h. These differences were maintained at 48 h and at 72 h. E accumulation by cultured GC was significantly reduced by > eightfold. There appeared a similar trend for reduced progesterone (P) in FF and decreased P production by GC in vitro. These results demonstrate that degenerative alterations in the DF indicative of atresia can be detected as early as 24 h after initiation of E 2 treatment; the index of atresia appearing earliest is a reduction in FF concentrations of E, and the first morphological changes in the DF can be observed 24 h later. This study indicates that biochemical alterations precede morphologic changes with E 2 ‐induced atresia, and should allow us to begin to determine the earliest events and putative initiation sites of atresia.