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Determining acrosomal status of the cynomolgus monkey ( Macaca fascicularis ) sperm by fluorescence microscopy
Author(s) -
Cross Nicholas L.,
Morales Patricio,
Fukuda Masaru,
Behboodi Esmail
Publication year - 1989
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/ajp.1350170205
Subject(s) - sperm , acrosome , acrosome reaction , antiserum , biology , andrology , agglutinin , fluorescence microscope , immunology , fluorescence , botany , lectin , medicine , antigen , physics , quantum mechanics
Abstract The acrosome of Macaca fascicularis sperm cannot be distinguished by conventional light microscopy, so determining whether sperm are acrosome‐intact or‐reacted is difficult. We describe methods for labeling the acrosomal region of sperm with two different probes: fluoresceinated Pisum sativum agglutinin and anti‐sperm antiserum. Acrosome‐intact sperm are much more heavily labeled in the acrosomal region than are acrosome‐reacted sperm, providing a simple means of differentiating the two types of sperm. The two probes detect similar numbers of acrosome‐reacted sperm following treatment with the divalent cation ionophore, A23187.