Accumulation of 2‐deoxy‐D‐glucose by primate oocytes fertilized in vitro | Zendy

Hutz R. J. | Zendy; Demayo F. J. | Zendy; Chan P. J. | Zendy; Dukelow W. R. | Zendy

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Accumulation of 2‐deoxy‐D‐glucose by primate oocytes fertilized in vitro

Author(s) -

Hutz R. J.,

Demayo F. J.,

Chan P. J.,

Dukelow W. R.

Publication year - 1984

Publication title -

american journal of primatology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.988

H-Index - 81

eISSN - 1098-2345

pISSN - 0275-2565

DOI - 10.1002/ajp.1350070108

Subject(s) - primate , oocyte , in vitro , squirrel monkey , biology , embryo , in vitro fertilisation , human fertilization , andrology , insulin , endocrinology , medicine , microbiology and biotechnology , anatomy , biochemistry , ecology

The present study was designed to investigate the accumulation of 2‐deoxy‐D‐glucose (2‐DG) by squirrel monkey oocytes fertilized in vitro; to assess the effects of insulin addition to the medium on 2‐DG accumulation; and, finally, to evaluate the use of 2‐DG in viability determinations of oocytes. Accumulation of 2‐DG by unfertilized oocytes from squirrel monkeys was 13.94 fmol/oocyte/3 h and was not affected by the addition of either 10 nM or 1 μM insulin. There was no change in 2‐DG accumulation with fertilization in vitro; 2‐DG accumulation by degenerate ova was reduced to background levels. These results suggest low utilization of glucose by early primate embryos similar to that demonstrated for other mammalian species; 2‐DG appears to be a good viability indicator of early primate embryos.

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