Premium
Single‐copy nuclear DNA sequences obtained from noninvasively collected primate feces
Author(s) -
Surridge Alison K.,
Smith Andrew C.,
BuchananSmith Hannah M.,
Mundy Nicholas I.
Publication year - 2002
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/ajp.1073
Subject(s) - biology , primate , genotyping , microsatellite , feces , nuclear dna , context (archaeology) , mitochondrial dna , locus (genetics) , evolutionary biology , dna sequencing , genetics , dna , zoology , gene , genotype , allele , ecology , paleontology
Noninvasively collected primate feces have been shown to provide a useful source of mitochondrial DNA for sequencing and nuclear microsatellite DNA for size analysis. In this study, single‐copy nuclear DNA sequences were obtained from noninvasively collected fecal samples of two species of wild tamarins, Saguinus fuscicollis and S. mystax , in the context of a project on the functional utility of color vision. Noninvasive genotyping of the X‐linked opsin gene is important for future studies of selection and adaptation at this locus in a number of primate species. The wide range of techniques that can now be applied successfully to DNA extracted from feces introduces a broad spectrum of potential genetic studies that can be undertaken on primates, without the need for intrusive or invasive methods. Am. J. Primatol. 56:185–190, 2002. © 2002 Wiley‐Liss, Inc.