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Characterization and screening of microsatellite loci in a wild lemur population ( Propithecus verreauxi verreauxi )
Author(s) -
Lawler Richard R.,
Richard Alison F.,
Riley Margaret A.
Publication year - 2001
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/ajp.1058
Subject(s) - biology , microsatellite , lemur , loss of heterozygosity , genetics , population , evolutionary biology , allele , zoology , gene , ecology , primate , demography , sociology
Sixteen dinucleotide microsatellite loci were isolated from the genome of Propithecus verreauxi verreauxi . All loci were polymorphic when genotyped on a minimum of 16 animals. The number of alleles across these loci ranges from two to 11. Additionally, seven of these loci were genotyped across a minimum of 200 animals in order to estimate heterozygosity and their potential for parentage assignment in this population. Using these seven loci, the mean heterozygosity in this population is 0.705, and the combined probability of these seven loci to exclude a random individual from parentage, when one parent is known, is 0.996. These data suggest that these loci will be useful for estimating a variety of population genetic and genealogical parameters in P. v. verreauxi populations. Am. J. Primatol. 55:253–259, 2001. © 2001 Wiley‐Liss, Inc.