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Inducible DNA Polymerase Chain Reaction Triggered by Oxidative Species
Author(s) -
Wang Sheng,
Zhao Jizhong,
Wang Li,
Zhang Jingwen,
Hu Hongmei,
Yu Ping,
Wang Rui
Publication year - 2021
Publication title -
asian journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.846
H-Index - 44
eISSN - 2193-5815
pISSN - 2193-5807
DOI - 10.1002/ajoc.202000377
Subject(s) - chemistry , dna , polymerase chain reaction , polymerase , base pair , dna polymerase , reactive oxygen species , thymidine , polymerase chain reaction optimization , microbiology and biotechnology , biophysics , biochemistry , gene , nested polymerase chain reaction , biology
We report here a chemically synthetic method to introduce a PinB‐modified thymidine into well‐defined DNA first. The modification was designed to disturb the T−A base‐pairing, thus regulate the DNA activity for PCR amplification. By treatment with small molecules, i. e., reactive oxygen species, the DNA polymerase activity was successfully restored. Due to tight temporal regulation and cell‐membrane permeability, our approach could lead to application potential in live cell.