Premium
4′,5′‐Bis(dimethylamino)fluorescein Exhibits pH‐Dependent Emission Behavior Distinct From That of Fluorescein
Author(s) -
Hwang Jun Yeon,
Shim Sangdeok,
Hwang Gil Tae
Publication year - 2018
Publication title -
asian journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.846
H-Index - 44
eISSN - 2193-5815
pISSN - 2193-5807
DOI - 10.1002/ajoc.201700577
Subject(s) - fluorescein , chemistry , fluorescence , photochemistry , optics , physics
4′,5′‐Diaminofluoresein ( DAF ) and 4′,5′‐bis(dimethylamino)fluorescein ( BDMAF ) have been synthesized and their pH‐dependent photophysical properties and protolytic equilibria investigated. Although the parent fluorescein exhibits decreased emission upon decreasing pH, DAF displayed quenched emission at all pH values; surprisingly, BDMAF exhibited strong fluorescence only at pH 3–6, with its emission almost completely quenched under strongly acidic, neutral, and basic conditions.