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Gene copy number variation in schizophrenia
Author(s) -
Sutrala Smitha R.,
Norton Nadine,
Williams Nigel M.,
Buckland Paul R.
Publication year - 2007
Publication title -
american journal of medical genetics part b: neuropsychiatric genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.393
H-Index - 126
eISSN - 1552-485X
pISSN - 1552-4841
DOI - 10.1002/ajmg.b.30645
Subject(s) - copy number variation , genetics , biology , gene , comparative genomic hybridization , gene dosage , disc1 , copy number analysis , computational biology , allele , genome , gene expression
Recent reports have highlighted the possibility that gene copy number variations play a role in the development of complex disorders and have suggested that some variations are very common in schizophrenic patients. We have carried out a comparative genomic hybridization screen using oligonucleotide probes of 891 candidate genes to look for very common copy number variance in schizophrenic patients. In addition we have developed a new approach for the detection and validation of putative copy number variation based upon established methods of allele quantification by DNA pooling and have used it to study 15 major candidates including dysbindin ( DTNBP1 ), neuregulin ( NRG1 ), RGS4 and DISC1 . With the exception of positive control sequences, no copy number variations were found for any of the genes in any samples by the use of either technique. Our data for the genes studied are in line with the known existence and frequency of CNVs as reported by recent large scale studies and suggest that gene copy number variations are not more common in schizophrenics than controls, although large ethnic differences cannot be excluded. © 2007 Wiley‐Liss, Inc.