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Possible role of preproghrelin gene polymorphisms in susceptibility to bulimia nervosa
Author(s) -
Ando Tetsuya,
Komaki Gen,
Naruo Tetsuro,
Okabe Kenjiro,
Takii Masato,
Kawai Keisuke,
Konjiki Fujiko,
Takei Michiko,
Oka Takakazu,
Takeuchi Kaori,
Masuda Akinori,
Ozaki Norio,
Suematsu Hiroyuki,
Denda Kenzo,
Kurokawa Nobuo,
Itakura Kotarou,
Yamaguchi Chikara,
Kono Masaki,
Suzuki Tatsuyo,
Nakai Yoshikatsu,
NishizonoMaher Aya,
Koide Masanori,
Murakami Ken,
Nagamine Kiyohide,
Tomita Yuichiro,
Ookuma Kazuyoshi,
Tomita Kazumi,
Tonai Eita,
Ooshima Akira,
Ishikawa Toshio,
Ichimaru Yuhei
Publication year - 2006
Publication title -
american journal of medical genetics part b: neuropsychiatric genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.393
H-Index - 126
eISSN - 1552-485X
pISSN - 1552-4841
DOI - 10.1002/ajmg.b.30387
Subject(s) - bulimia nervosa , linkage disequilibrium , haplotype , genetics , anorexia nervosa , eating disorders , medicine , allele , genotype , endocrinology , biology , gene , psychiatry
Previous investigations have suggested that ghrelin, an endogenous orexigenic peptide, is involved in the pathology of eating disorders. We conducted a study to determine whether any preproghrelin gene polymorphisms are associated with eating disorders. Three hundred thirty‐six eating disorder patients, including 131 anorexia nervosa (AN)‐restricting types (AN‐R), 97 AN‐binge eating/purging types (AN‐BP) and 108 bulimia nervosa (BN)‐purging types (BN‐P), and 300 healthy control subjects participated in the study. Genotyping was performed to determine the polymorphisms present, and with this information, linkage disequilibrium (LD) between the markers was analyzed and the distributions of the genotypes, the allele frequencies, and the haplotype frequencies were compared between the groups. The Leu72Met (408 C > A) (rs696217) polymorphism in exon 2 and the 3056 T > C (rs2075356) polymorphism in intron 2 were in LD (D′ = 0.902, r 2 = 0.454). Both polymorphisms were significantly associated with BN‐P (allele‐wise: P = 0.0410, odds ratio (OR) = 1.48; P = 0.0035, OR = 1.63, for Leu72Met and 3056 T > C, respectively). In addition, we observed a significant increase in the frequency of the haplotype Met72‐3056C in BN‐P patients ( P = 0.0059, OR = 1.71). Our findings suggest that the Leu72Met (408 C > A) and the 3056 T > C polymorphisms of the preproghrelin gene are associated with susceptibility to BN‐P. © 2006 Wiley‐Liss, Inc.