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A novel PIGN mutation and prenatal diagnosis of inherited glycosylphosphatidylinositol deficiency
Author(s) -
Nakagawa Taku,
TaniguchiIkeda Mariko,
Murakami Yoshiko,
Nakamura Shota,
Motooka Daisuke,
Emoto Tomomi,
Satake Wataru,
Nishiyama Masahiro,
Toyoshima Daisaku,
Morisada Naoya,
Takada Satoshi,
Tairaku Shinya,
Okamoto Nobuhiko,
Morioka Ichiro,
Kurahashi Hiroki,
Toda Tatsushi,
Kinoshita Taroh,
Iijima Kazumoto
Publication year - 2016
Publication title -
american journal of medical genetics part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.064
H-Index - 112
eISSN - 1552-4833
pISSN - 1552-4825
DOI - 10.1002/ajmg.a.37397
Subject(s) - proband , exome sequencing , genetics , mutation , compound heterozygosity , exon , biology , exome , genetic counseling , gene
Glycosylphosphatidylinositol (GPI) anchors tether proteins to the extracellular face of eukaryotic plasma membranes. Defects in the human GPI anchor biosynthetic pathway cause inherited GPI deficiencies (IGDs) characterized by multiple congenital anomalies: dysmorphic faces, developmental delay, hypotonia, and epilepsy. We report the case of a 6‐year‐old boy with severe psychomotor developmental delay, epilepsy, and decreased granulocyte surface expression of GPI‐anchored protein that suggested autosomal recessive GPI deficiency. The case underwent target exome sequencing to screen for IGDs. Target exome sequencing of the proband identified an apparently homozygous c.808T > C (p.Ser270Pro) mutation in PIGN , a gene involved in the GPI anchor biosynthetic pathway. As his parents were expecting another child, genetic carrier screening was conducted for the parents. Direct sequencing of the parents identified a heterozygous c.808T > C PIGN mutation in the father but none in the mother. To identify the mother's mutation, we performed semi‐quantitative real‐time PCR of the PIGN exons and long PCR, identifying a microdeletion in PIGN (del exons 2–14). The proband had inherited this microdeletion from his mother. Prenatal diagnosis of the fetus revealed that it was a heterozygous carrier of the mother's pathogenic allele. Here, we report a sporadic case of inherited GPI deficiency with a PIGN mutation and the first case of prenatal diagnosis for GPI deficiency. © 2015 Wiley Periodicals, Inc.

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