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Gene‐targeted deletion of OPCML and Neurotrimin in mice does not yield congenital heart defects
Author(s) -
Ye Maoqing,
Parente Fabienne,
Li Xiaodong,
Perryman M. Benjamin,
Zelante Leopoldo,
WynshawBoris Anthony,
Chen Ju,
Grossfeld Paul
Publication year - 2014
Publication title -
american journal of medical genetics part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.064
H-Index - 112
eISSN - 1552-4833
pISSN - 1552-4825
DOI - 10.1002/ajmg.a.36441
Subject(s) - penetrance , phenotype , biology , haploinsufficiency , genetics , gene , chromosomal translocation , breakpoint , exon , population , medicine , environmental health
Jacobsen syndrome (11q‐) is a rare chromosomal disorder caused by deletions in distal11q. Many of the most common and severe congenital heart defects that occur in the general population occur in 11q‐. Previous studies have demonstrated that gene‐targeted deletion in mice of ETS‐1, a cardiac transcription factor in distal 11q, causes ventricular septal defects with 100% penetrance. It is unclear whether deletion of other genes in distal 11q contributes to the full spectrum of congenital heart defects that occur in 11q‐. Three patients with congenital heart defects have been identified that carry a translocation or paracentric inversion with a breakpoint in distal 11q disrupting one of two functionally related genes, OPCML and Neurotrimin . OPCML and Neurotrimin are two members of the IgLON subfamily of cell adhesion molecules. In this study, we report the generation and cardiac phenotype of single and double heterozygous gene‐targeted OPCML and Neurotrimin knockout mice. No cardiac phenotype was detected, consistent with a single gene model as the cause of the congenital heart defects in 11q‐. © 2014 Wiley Periodicals, Inc.