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Effect of sample holding, cryopreservation, and storage on the human lymphocyte cytogenetic test
Author(s) -
Tomkins Darrell J.,
Scheid Elizabeth E.
Publication year - 1986
Publication title -
american journal of industrial medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 104
eISSN - 1097-0274
pISSN - 0271-3586
DOI - 10.1002/ajim.4700090410
Subject(s) - medicine , cryopreservation , test (biology) , lymphocyte , immunology , sample (material) , andrology , genetics , embryo , biology , chromatography , paleontology , chemistry
In monitoring occupational populations with the human lymphocyte cytogenetic test, it is not always possible to collect and process matched samples on the same day, even though this would be desirable to control for technical variables. The effects of holding samples for 24 hours at 4°C and 22°C, freezing lymphocytes in dimethylsulfoxide at −180°C, and keeping fixed cells for 6 days at 4°C before slide‐making were examined. Final cell count, mitotic index, percentage of cells in first division, percentage of cells with chromosomal aberrations, and sister chromatid exchange per cell were measured in paired cultures. A significant increase in the frequency of cells with chromatid breaks occurred after prolonged fixation, so all other results were obtained from freshly fixed cells. Although holding at 22°C and cryopreservation had significant effects on the mitotic index and entry of lymphocytes into the cell division cycle, the cytogenetic endpoints were not affected by any of the sample manipulations. Thus, samples can be held at 4°C or 22°C for 24 hours, or frozen lymphocytes can be stored for at least a week, without altering the cytogenetic endpoints.