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The in vitro cytotoxicity of asbestos fibers: I. P388D 1 cells
Author(s) -
Wright A.,
Cowie H.,
Gormley I. P.,
Davis J. M. G.
Publication year - 1986
Publication title -
american journal of industrial medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 104
eISSN - 1097-0274
pISSN - 0271-3586
DOI - 10.1002/ajim.4700090409
Subject(s) - cytotoxicity , chrysotile , lactate dehydrogenase , incubation , in vitro , medicine , viability assay , asbestos , cytotoxic t cell , intracellular , toxicology , enzyme , biochemistry , biology , materials science , composite material
In this study, the cytotoxicity of 13 fibrous samples of known fiber number and dimensions has been established in P388D 1 cells. The cells were exposed in vitro to dust concentrations of 10 or 50 μg/ml and, after incubation for 24 or 48 hours, any changes in cellular viability, lactate dehydrogenase, and glucosaminidase levels were determined. In general, there was a close association between the reduction in cellular viability and the loss of intracellular enzymes induced by each dust, the chrysotile asbestos samples proving more cytotoxic than the amphiboles. The cytotoxicity of the fibrous dusts was shown to be related to the number of fibers greater than 8 μm in length in the samples.