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External validation of recombinant antigens for serodiagnosis of machine operator's lung
Author(s) -
Barrera Coralie,
Reboux Gabriel,
Warfolomeow Isabel,
Rog Bénédicte,
Millon Laurence,
Roussel Sandrine
Publication year - 2014
Publication title -
american journal of industrial medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 104
eISSN - 1097-0274
pISSN - 0271-3586
DOI - 10.1002/ajim.22270
Subject(s) - recombinant dna , hypersensitivity pneumonitis , antigen , medicine , serology , virology , immunology , lung , biology , biochemistry , antibody , gene
Background Machine operator's lung (MOL) is a hypersensitivity pneumonitis the diagnosis of which is difficult. Our laboratory previously developed an ELISA test using recombinant antigens from Mycobacterium immunogenum isolated in French plant. The objective was to validate the previous ELISA results with ten new suspected cases from Germany. Methods Two serological analyses were performed: ELISA with the six recombinant antigens, and electrosyneresis with crude antigens of M. immunogenum and three other main species isolated from contaminated metalworking fluids. Results The two recombinant antigens acyl‐CoA dehydrogenase and dihydrolipoyl dehydrogenase, combined together, and electrosyneresis are useful in making the diagnosis regardless of the clinical and radiological data. Finally 9 out of the 10 suspected cases were declared as MOL. Conclusions Despite the geographical distance, the crude and recombinant antigens produced to investigate the clustered French cases also proved to be useful in diagnosing the suspected cases in Germany. Am. J. Ind. Med. 57:195–201, 2014. © 2013 Wiley Periodicals, Inc.