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Influence of the delta‐aminolevulinic acid dehydratase (ALAD) polymorphism on biomarkers of lead exposure in Turkish storage battery manufacturing workers
Author(s) -
Süzen H. Sinan,
Duydu Yalçın,
Aydın Ahmet,
Işımer Aşkın,
Vural Nevin
Publication year - 2003
Publication title -
american journal of industrial medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 104
eISSN - 1097-0274
pISSN - 0271-3586
DOI - 10.1002/ajim.10158
Subject(s) - genotype , dehydratase , medicine , restriction fragment length polymorphism , porphobilinogen synthase , urinary system , polymorphism (computer science) , gene polymorphism , lead poisoning , endocrinology , gene , biochemistry , chemistry , psychiatry
Background The relationship between delta‐aminolevulinic acid dehydratase polymorphism (ALAD) and biomarkers of exposure was investigated in Turkish lead workers in this study. Methods Seventy two male lead battery manufacturing workers were selected for the study. Blood lead (BPb) and urinary lead (UPb) concentrations were determined by atomic absorption spectrometry. Erythrocyte ALAD activity and urinary 5‐aminolevulinic acid (UALA) were measured spectrophotometrically. The polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) technique was used to determine the genotype of the ALAD gene. Results In total, 51 workers (70.8%) had the ALAD 1‐1 genotype, whereas 21 workers (29.2%) had the ALAD 1‐2 genotype. No significant relationships were found between the two genotypes and BPb, UPb, and ALAD activity. ALAD1 homozygotes showed significantly higher levels of UALA in comparison with those ALAD2 carriers. Conclusions ALAD 1‐1 individuals might be an increased risk compared to ALAD2 carriers to disturbance in heme biosynthetic pathway in high lead exposure. Am. J. Ind. Med. 43: 165–171, 2003. © 2003 Wiley‐Liss, Inc.