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Cholinesterase measurements with an automated kit
Author(s) -
Oliveira Georgino H.,
Henderson John D.,
Wilson Barry W.
Publication year - 2002
Publication title -
american journal of industrial medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 104
eISSN - 1097-0274
pISSN - 0271-3586
DOI - 10.1002/ajim.10105
Subject(s) - acetylcholinesterase , medicine , cholinesterase , aché , enzyme , chromatography , toxicology , biochemistry , chemistry , biology
Background The Test‐mate kit determines acetylcholinesterase (AChE, EC 3.1.1.7) and hemoglobin content of a drop of blood, displaying enzyme activities normalized to 25°C. Previous models produced inconsistent results at different temperatures. This report focuses on the current model, ChE 400, and two instruments of a previous OP model. Methods AChE activities were determined by the Ellman assay, using the three kits and a 96‐well microplate reader. Temperatures ranged from 10 to 37°C. Fetal bovine serum was the source of AChE. Results Normalized activities decreased below 20°C in the ChE model and below 25°C in the OP models. Activities of the same serum sample differed between the three Test‐mate kits, ranging from 1.03 to 1.49 μmoles/min/ml. Percent errors were greater than with the microplate reader at all temperatures. Conclusions Neither we nor the manufacturer recommend the current Test‐mate model for fieldwork. Nevertheless, there have been field measurements with Test‐Mate kits, and we recommend that an enzyme activity standard be run in parallel with their use. Am. J. Ind. Med. Suppl. 2:49–53, 2002. © 2002 Wiley‐Liss, Inc.