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Mitochondrial lineage A2ah found in a pre‐Hispanic individual from the Andean region
Author(s) -
Russo M. G.,
Dejean C. B.,
Avena S. A.,
Seldes V.,
Ramundo P.
Publication year - 2018
Publication title -
american journal of human biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.559
H-Index - 81
eISSN - 1520-6300
pISSN - 1042-0533
DOI - 10.1002/ajhb.23134
Subject(s) - lineage (genetic) , mitochondrial dna , evolutionary biology , context (archaeology) , haplotype , geography , gene flow , biology , genetics , gene , archaeology , genetic variation , genotype
Objectives The aim of this study was to contribute to the knowledge of pre‐Hispanic Andean mitochondrial diversity by analyzing an individual from the archaeological site Pukara de La Cueva (North‐western Argentina). The date of the discovery context (540 ± 60 BP) corresponds to the Regional Developments II period. Methods Two separate DNA extractions were performed from dentin powder of one tooth. HVR I was amplified by PCR from each extract in three overlapping fragments and the haplotype was determined by consensus among all obtained sequences. The procedures were carried out under strict protocols developed for working with ancient DNA. Results The individual belonged to the A2ah lineage due to the presence of the 16097C and 16098G transitions, which constitute its distinctive motif. This lineage is very rare in Native American populations and was described in four individuals from current groups inhabiting the Bolivian Llanos, two from South‐eastern Brazil, and one from the Gran Chaco region. In addition, two other mutations (16260T and 16286T) were shared with one of the individuals from the Bolivian Llanos region. Conclusions Considering that the origin of this lineage was postulated for the South American lowlands, the present pre‐Hispanic discovery in the Andean area could be taken as a new evidence of gene flow between these regions. Also, it allows the questioning of the geographical origin of this mitochondrial lineage.