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Polymorphism of LRP5 , but not of TNFRSF11B , is associated with a decrease in bone mineral density in postmenopausal maya‐mestizo women
Author(s) -
CantoCetina Thelma,
Polanco Reyes Lucila,
González Herrera Lizbeth,
RojanoMejía David,
CoralVázquez Ramón Mauricio,
Coronel Agustín,
Canto Patricia
Publication year - 2013
Publication title -
american journal of human biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.559
H-Index - 81
eISSN - 1520-6300
pISSN - 1042-0533
DOI - 10.1002/ajhb.22464
Subject(s) - bone mineral , single nucleotide polymorphism , femoral neck , genotype , linkage disequilibrium , osteoporosis , genotyping , haplotype , genetics , lrp5 , medicine , bone density , population , biology , gene , wnt signaling pathway , environmental health
Objective Osteoporosis is a complex disease characterized principally by low bone mineral density (BMD), which is determined by an interaction of genetic, metabolic, and environmental factors. The aim of this study was to analyze the possible association among one polymorphism of LRP5 and three polymorphisms of TNFRSF11B as well as their haplotypes with BMD variations in Maya‐Mestizo postmenopausal women. Methods We studied 583 postmenopausal women of Maya‐Mestizo ethnic origin. A structured questionnaire for risk factors was applied and BMD was measured in lumbar spine (LS), total hip (TH), and femoral neck (FN) by dual‐energy X‐ray absorptiometry. DNA was obtained from blood leukocytes. One single‐nucleotide polymorphism of LRP5 (rs3736228, p.A1330V) and three of TNFRSF11B (rs4355801, rs2073618, and rs6993813) were studied using real‐time PCR allelic discrimination for genotyping. Differences between the means of the BMDs according to the genotype were analyzed with covariance. Deviations from Hardy–Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r 2 , and haplotype analysis of TNFRSF11B was conducted. Results The Val genotype of the rs3736228 (p.A1330V) of LRP5 was significantly associated with BMD variations at the LS, TH, and FN. None of the three polymorphisms of TNFRSF11B was associated with BMD variations. Conclusions Our results show that p.A1330V was significantly associated with BMD variations at all three skeletal sites analyzed; the Val allele and the Val/Val genotype were those most frequently found in our population. Am. J. Hum. Biol. 25:713–718, 2013. © 2013 Wiley Periodicals, Inc.