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Duffy blood group genotyping in French Basques using polymerase chain reaction with allele‐specific primers (PCR‐ASP)
Author(s) -
Bauduer Frédéric,
Touinssi Mhammed,
Degioanni Anna,
Leroux Stéphane,
Dutour Olivier,
Ducout Louis,
De Micco Philippe,
Chiaroni Jacques
Publication year - 2003
Publication title -
american journal of human biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.559
H-Index - 81
eISSN - 1520-6300
pISSN - 1042-0533
DOI - 10.1002/ajhb.10230
Subject(s) - genotyping , polymerase chain reaction , allele , biology , allele frequency , population , genetics , hemagglutination , genotype , demography , antigen , gene , sociology
Abstract Blood group antigens such as Duffy represent interesting models for population genetics studies. The distribution of the Duffy blood group was determined using PCR in a sample of Basque (n = 126) and non‐Basque (n = 110) patients from the general hospital of the French Basque Country. The frequency of FY*A allele was significantly lower among autochthonous French Basques ( P < 0.001). This result, obtained for the first time by PCR analysis in this population, was within the range of previous historical studies in various Basque subpopulations using traditional hemagglutination methods. When compared with European data, our Basque sample demonstrated the lowest FY*A/FY*B ratio, this fact confirming hemotypology data published before the molecular biology era. Our results with FY*X were quite similar to those reported in European populations. Am. J. Hum. Biol. 16:78–81, 2004. © 2003 Wiley‐Liss, Inc.

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