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Rearrangement and expression of MDM 2 oncogene in chronic lymphocytic leukemia
Author(s) -
Huang Yao Qi,
Raphael Bruce,
Buchbinder Aby,
Li Jian Jun,
Zhang Wei Gue,
FriedmanKien Alvin E.
Publication year - 1994
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830470215
Subject(s) - chronic lymphocytic leukemia , pathogenesis , peripheral blood mononuclear cell , trisomy , biology , northern blot , cancer research , southern blot , leukemia , microbiology and biotechnology , chromosome , chromosome abnormality , gene , gene expression , immunology , genetics , karyotype , in vitro
Since trisomy 12 is the most common chromosome abnormality found in CLL and MDM 2 has been mapped to this chromosome, we examined the possible association of MDM 2 in the pathogenesis of CLL. A rearrangement of the MDM 2 gene was observed in 4 of 11 peripheral blood mononuclear cells (PBMC) from patients with CLL by Southern biot hybridization. Expression of MDM 2 was detected in all of the CLL samples examined by Northern blot. However, neither gross amplification nor overexpression of the MDM 2 gene was found in CLL. The data suggest that MDM 2 may play a role in the pathogenesis of CLL and may help to explain how abnormalities of chromosome 12 are related to CLL. © 1994 Wiley‐Liss, Inc.