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IL‐5 mRNA expression in blood lymphocytes from patients with Kimura's disease and parasite infection
Author(s) -
Enokihara Hideo,
Koike Toshiyuki,
Arimura Hironari,
Aoyagi Masakuni,
Watanabe Kanji,
Nakamura Yuichi,
Yamashiro Kyoko,
Tsuruoka Nobuo,
Tsujimoto Masafumi,
Saito Kenji,
Furusawa Shimpei,
Shishido Hideo
Publication year - 1994
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830470202
Subject(s) - eosinophilia , immunology , in vivo , reverse transcriptase , biology , lymphokine , messenger rna , immunopathology , in vitro , lymphocyte , interleukin 5 , polymerase chain reaction , interleukin , medicine , microbiology and biotechnology , immune system , cytokine , gene , biochemistry
Blood lymphocytes from patients with eosinophilia are known to produce interleukin‐5 (IL‐5) with appropriate stimulation in vitro. To determine whether blood lymphocytes from these patients produce IL‐5 in vivo, we tested the IL‐5 mRNA expression in blood lymphocytes immediately after separation by reverse‐transcriptase polymerase chain reaction (RT‐PCR) method. We found that lymphocytes from eosinophilic patients expressed IL‐5 mRNA, but lymphocytes from normal volunteers did not express the lymphokine. These findings suggest that in patients with eosinophilia, peripheral blood lymphocytes produce IL‐5 in vivo. © 1994 Wiley‐Liss, Inc.