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Increased binding of SP1 to the γ‐globin gene promoter upon site‐specific cytosine methylation
Author(s) -
Sengupta Pritam K.,
Lavelle Donald,
Desimone Joseph
Publication year - 1994
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830460302
Subject(s) - methylation , cpg site , cytosine , microbiology and biotechnology , enhancer , dna methylation , biology , promoter , epigenetics of physical exercise , binding site , transcription (linguistics) , repressor , gene , genetics , transcription factor , gene expression , linguistics , philosophy
We have studied the effect of site‐specific cytosine methylation on the binding of nuclear proteins to the γ‐globin promoter region from −71 to −34. This sequence was recently shown to contain a stage selector element responsible for increased transcription from the γ compared to the β promoter when both are linked to Locus Control Region enhancer sequences. We observed that Sp1 binding to this sequence is increased upon site‐specific cytosine methylation, such that only methylation of the cytosine in the −50 CpG dinucleotide effects Sp1 binding. Methylation of the distal C of the Hpall site has no effect. The possible functional role of Sp1 as a repressor of γ‐globin synthesis is discussed. © 1994 Wiley‐Liss, Inc.