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Lineage determination of CD7+ CD5− CD2− and CD7+ CD5+ CD2− lymphoblasts: Studies on phenotype, genotype, and gene expression of myeloperoxidase, CD3ϵ, and CD3δ
Author(s) -
Yoneda Noriko,
Tatsumi Eiji,
Teshigawara Keisuke,
Nagata Shigekazu,
Nagano Takahiro,
Kishimoto Yuji,
Kimura Takashi,
Yasunaga Koujiro,
Yamaguchi Nobuo
Publication year - 1994
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830450408
Subject(s) - cd5 , cd3 , cd8 , t cell receptor , microbiology and biotechnology , biology , gene rearrangement , t cell , antigen , immunology , gene , genetics , immune system
Abstract The gene expression of myeloperoxldase (MPO), CD3ϵ and CD3δ molecules, the gene rearrangement of T‐cell receptor (TCR)δ, γ, and β and Immunoglobulin heavy (IgH) chain, and the expression of cell‐surface antlgens were investigated In seven cases of CD7+ CD5− CD2− and four cases of CD7+ CD5+ CD2− acute lymphoblastic leukemia or lymphoblastic lymphoma (ALL/LBL) blasts, which were negative for cytochemical myeloperoxidase (cyMPO). More mature T‐lineage blasts were also investigated in a comparative manner. In conclusion, the CD7+ CD5− CD2− blasts included four categories: Undifferentiated blasts without lineage commitment, T‐lineage blasts, T‐/myeloid lineage blasts, and cyMPO‐negative myeioblasts. The CD7+ CD5+ CD2− blasts included two categories; T‐lineage and T‐/myeloid lineage blasts. The 11 cases were of the germ‐line gene (G) for TCRβ and IgH. Four cases were G for TCRδ and TCRγ. The others were of the monoclonally rearranged gene (R) for TCRδ and G for TCRγ or R for both TCRδ and TCRγ. The expression or In vitro induction of CD13 and/or CD33 antigens correlated with the immaturity of these neoplastic T cells, since it was observed In all 11 CD7+ CD5− CD2−and CD7+ CD5+ CD2−, and some CD7+ CD5+ CD2+ (CD3− CD4− COB‐) cases, but not in CD3± CD4+ CD8+ or CD3+ CD4+ CD8− cases. CD3ϵ mRNA, but not CD3δ mRNA, was detected in two CD7+ CD5− CD2− cases, while mRNA of neither of the two CD3 moleculea was detected In the other tested CD7+ CD5−CD2− cases. In contrast, mRNA of both CD3e and CD36 were detected in ail CD7+ CD5+ CD2− cases, indicating that CD7+ CD5+ CD2− blasts at least belong to T‐lineage. The blasts of two CD7+ CD5− CD2− cases with entire germ‐line genes and without mRNA of the three molecules (MPO, CD3ϵ, and CD3δ) were regarded as being at an undifferentiated stage prior to their commitment to either T‐ or myelold‐lineage. The co‐expression of the genes of MPO and CD3e In a CD7+ CD5− CD2− case or MPO, CD3ϵ, and CD3δ in a CD7+ CD5+ CD2− case suggested the presence of some overlapping phase for T‐ and myeloid‐lineage commitment during Immature stages of differentiation. This heips understand the conversion of some T‐ALL/LBL cases to acute myeloblastic leukemia (AML). The detection of CD3ϵ, but not CD3δ mRNA, in two CD7+ CD5− CD2−cases indicated that the gene expression of CD3ϵ occurs at a more immature stage of differentiation than that of the CD3δ chain. © 1994 Wiley‐Liss, Inc.