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Expression, of the thrombospondin receptor (CD36) on the cell surface in megakaryoblastic and promegakaryocytic leukemias: Increment of the receptor by megakaryocyte differentiation in vitro
Author(s) -
Imamura Nobutaka,
Ota Hiromi,
Abe Kazuhiro,
Kuramoto Atsushi
Publication year - 1994
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830450216
Subject(s) - megakaryocyte , acute megakaryoblastic leukemia , antigen , thrombospondin , leukemia , monoclonal antibody , cd36 , cluster of differentiation , platelet , microbiology and biotechnology , biology , immunology , chemistry , receptor , cell , antibody , haematopoiesis , biochemistry , stem cell , metalloproteinase , matrix metalloproteinase
In this report we describe four cases of acute megakaryocytic leukemia demonstrated by the presence of megakaryocyteplatelet‐related cell‐surface antigens. These were detected utilizing flow cytometry and monoclonal antibodies in addition to both platelet peroxidase activity, which was shown by ultrastractural cytochemistry, and emergence of differentiation antigens, while culturing these leukemic cells. The blasts of one patient possessed both platelet Gplb and Gpllb/llla cell‐surface antigens detected by AN51(CD42b), J15, P2, and HPL2(CD41), respectively, whereas the remaining three patients almost completely lacked Gplb cell‐surface antigen. Hence the former were diagnosed as immature(pro)megakaryocytic leukemia and the latter as acute megakaryoblastic leukemia from the viewpoint of immunophenotypic analysis. While we cultured these leukemic cells in conditioned medlum prepared from phytohemagglutinin‐stimulated leukocytes and interleukin 3, expression of CD36(OKM5) antigen (thrombospondin receptor) increased gradually according to the differentiation and maturation of these cells. Finally, all leukemic cells differentiated to mature megakaryocytes. The function of CD36 on these cells remains to be elucidated. © 1994 Wiley‐Liss, Inc.