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Von willebrand factor availability in platelet concentrates stored for 5 days
Author(s) -
Cesar Jesús M.,
GarciaAvello Angel,
Monteagudo Juán,
Espinosa José I.,
Lodos José C.,
Castillo Ricardo,
Navarro José L.
Publication year - 1994
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830450203
Subject(s) - von willebrand factor , platelet , chemistry , ristocetin , basal (medicine) , bleeding time , blood preservation , platelet aggregation , medicine , endocrinology , immunology , andrology , diabetes mellitus
Von Willebrand factor (vWF) availability was assessed in platelet concentrates (PCs). After 5 days of storage, 82 ± 9% of basal levels of ristocetin cofactor activity (vWF:RCo) remained in PCs. vWF antigen (vWF:Ag) increased up to 166 ± 38% ( P < 0.05) In the same period. Autoradiograph pattern of vW:Ag showed an increase in low molecular weight multimers, and fast migrating multimeric forms were visualized by crossed immunoeiectrophoresis on day 5. Studies carried out in platelet free plasma stored as PCs showed similar changes in vWF:RCo but increments in vWF:Ag were not detected. These data indicate that PCs maintain vWF:RCo levels of clinical value even after 5 days of storage and suggest that vWF comes out from platelets to plasma during storage. © 1994 Wiley‐Liss, Inc.