Premium
Variation in DNA content of immature normal bone marrow cells
Author(s) -
Widell S.,
Auer G.,
Hast R.,
Reizenstein P.
Publication year - 1993
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830430412
Subject(s) - coefficient of variation , flow cytometry , dna , bone marrow , aneuploidy , staining , andrology , microbiology and biotechnology , biology , pathology , immunology , chemistry , genetics , medicine , chromosome , gene , chromatography
A variability in DNA content detected was found with image cytometry, in immature bone marrow cells from 13 healthy donors (median age 31 yr). The mean coefficient of variation (C.V.) of the DNA content was found to be significantly ( P = 0.0002) higher in immature blasts and promyelocytes than in mature granulocytes, lymphocytes, and monocytes. The finding was not due to high DNA contents secondary to DNA‐synthesis in immature cells, since the percentage of such cells with a reduced DNA content was also significantly ( P = 0.0424‐0.0002) increased. The error of the method expressed as the variance of multiple measurements has been found to be 0.0002‐0.0004. A staining error has been found for some hydrolysis times, but not for times between 60 and 120 minutes. A measuring error was found for an average of 1.38 to 3.38% of the cells.