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Heterogeneous proliferative effect of tumor necrosis factor‐α and lymphotoxin on mitogen‐activated B cells from B‐chronic lymphocytic leukemia
Author(s) -
AlvarezMon M.,
GarciaSuarez J.,
Prieto A.,
Manzano L.,
Reyes E.,
Lorences C.,
Peraile G.,
Jorda J.,
Durantez A.
Publication year - 1993
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830430202
Subject(s) - lymphotoxin , lymphotoxin beta receptor , tumor necrosis factor alpha , chronic lymphocytic leukemia , immunology , lymphotoxin alpha , leukemia , in vitro , medicine , biology , cancer research , biochemistry
The proliferative effect of tumor necrosis factor‐α (TNF‐α) and lymphotoxin on B cells from patients with B‐chronic lymphocytic leukemia (B‐CLL) was studied. Fresh purified B‐CLL lymphocytes showed no proliferative response to either recombinant (r) TNF‐α or r‐lymphotoxin. However, after 'in vitro' activation of B‐CLL lymphocytes for 2 days with Staphylococcus aureus Cowan 1 (SAC), four of seven patients showed enhanced blastogenic response in the presence of either rTNF‐α or r‐Iymphotoxin. We also found that the proliferative response of SAC‐activated B‐CLL lymphocytes to the two cytokines was independent of that found in the presence of interleukin‐2. These results demonstrate that TNF‐α and lymphotoxin can heterogeneously support the proliferation of in vitro activated B cells from B‐CLL patients and may reflect the biological heterogeneity of B‐CLL disease.