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Long‐term high‐level expression of human β‐globin occurs following transplantation of transgenic marrow into irradiated mice
Author(s) -
Himelstein Andrew,
Ward Maureen,
Podda Silvio,
de la Flor Weiss Eduardo,
Costantini Frank,
Bank Arthur
Publication year - 1993
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830420304
Subject(s) - transgene , bone marrow , biology , genetically modified mouse , transplantation , microbiology and biotechnology , genetic enhancement , gene expression , globin , gene , cancer research , immunology , medicine , genetics
Abstract When the human β‐globin gene is transferred into the bone marrow cells of live mice, its expression is very low. To investigate the reason for this, we transferred the bone marrow of transgenic mice containing and expressing the human β‐globin into irradiated recipients. We demonstrate that long‐term high level expression of the human β‐globin gene can be maintained in the marrow and blood of irradiated recipients following transplantation. Although expression decreased over time in most animals because of host marrow reconstitution, the ratio of human β‐globin transgene expression to endogenous mouse β‐globin gene expression in donor‐derived erythroid cells remained constant over time. We conclude that there is no inherent limitation to efficient expression of an exogenous human β‐globin gene in mouse bone marrow cells following marrow transplantation. © 1993 Wiley‐Liss, Inc.