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Interleukin‐2 production by primary adult T cell leukemia tumor cells is macrophage dependent
Author(s) -
Arima Naomichi,
Daitoku Yasuhisa,
Hidakia Shiroh,
Matsushita Kakushi,
Ohtsubo Hideo,
Fukumori Junko,
Tanaka Hiromitsu,
Yamamoto Yusei,
Fujimoto Kouji,
Onoue Kaoru
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830410407
Subject(s) - macrophage , autocrine signalling , endogeny , biology , cell culture , cancer research , immunology , leukemia , microbiology and biotechnology , in vitro , endocrinology , biochemistry , genetics
We have investigated the cellular requirements for IL‐2 production by autocrine proliferating tumor cells from four patients with adult T cell leukemia (ATL). Cultures of these ATL cells both produced endogenous IL‐2 protein in the absence of added mitogen and proliferated at higher levels when exogenous recombinant IL‐2 was added. Depletion of macrophages in the tumor cell cultures resulted in a sharp decline in tumor cell IL‐2 production, while re‐addition of macrophages reconstituted this response. Macrophage‐derived factors including IL‐6 and IL‐1 also reconstituted IL‐2 production in these macrophage depleted cultures. These results raise the possibility that macrophages may play a central role in HTLV‐I mediated immortalization of T cells. © 1992 Wiley‐Liss, Inc.