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IgD myeloma presenting as a testicular tumor: Establishment and characterization of an IgD‐secreting myeloma cell line
Author(s) -
Ishii Kazuyoshi,
Yamato Kenji,
Kubonishi Ichiro,
Taguchi Hirokuni,
Ohtsuki Yuji,
Miyoshi Isao
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830410314
Subject(s) - immunoglobulin d , immunoglobulin light chain , bone marrow , cd19 , biology , cancer research , multiple myeloma , immunology , pathology , antibody , medicine , b cell
A 72‐year‐old man presented with a left testicular tumor and underwent orchiectomy. The tumor was massively infiltrated with myeloma cells bearing monoclonal cytoplasmic IgDλ. Three months after orchiectomy, he developed huge abdominal masses and subsequently ascites containing numerous myeloma cells. An IgD‐secreting myeloma cell line, designated delta‐47, was established from the ascites. This cell line expressed CD4 and CD38, but lacked Fc and complement receptors, surface immunoglobulin, CD19, HLA‐DR, and PCA‐1. CD30 was detected on the cultured cells but not on the ascites tumor cells. Delta‐47 cells secreted the same immunoglobulin (IgDλ) as was found in the patient's serum. The light chain had a molecular weight of 35 kD which was larger than that of the normal light chain. Chromosome analysis of delta‐47 revealed an aneuploid karyotype with complex abnormalities including 1q+, 2p+, and 14q+. To our knowledge, this is the only IgD‐secreting myeloma cell line and would provide a useful tool for the study of IgD production and IgD myeloma. © 1992 Wiley‐Liss, Inc.