z-logo
Premium
Influence of calcium permeabilization and membrane‐attached hemoglobin on erythrocyte deformability
Author(s) -
Friederichs E.,
Farley R. A.,
Meiselman H. J.
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830410306
Subject(s) - ionophore , calcium , hemoglobin , erythrocyte deformability , chemistry , membrane , biophysics , red blood cell , population , erythrocyte membrane , red cell , calcium in biology , intracellular , biochemistry , biology , medicine , environmental health , organic chemistry
The present study was designed to evaluate the influence of intracellular calcium [Ca] 1 regulated membrane attached hemoglobin (Hb m ) on the deformability of human RBC and ghosts. [Ca] 1 of RBC was elevated via the Ionophore A23187 (10 μM); the deformability of RBC and resealed ghosts was determined via measuring RBC and ghost transit times through 5 μm diameter pores with the Cell Transit Analyzer (CTA). Salient results included: (1) significantly increased RBC levels of Hb m following ionophore treatment; (2) elevated Hb m with increasing lysing medium calcium concentration (0–5 mM); (3) decreased deformability of both intact RBC and ghosts with increasing Hb m and significant (P < 0.02 or better) linear relationships between Hb m and RBC or ghost transit times; and (4) an increased sensitivity to ionophore treatment/membrane attached hemoglobin for the higher percentiles of the CTA transit time distribution (i.e., for more rigid subpopulations). Our results thus indicate that calcium‐induced interaction of hemoglobin with the RBC membrane produces cellular rheological changes; in addition, they demonstrate the usefulness of the CTA system in measuring both average RBC rheologic behavior and the distribution of cellular rheologic properties within an erythrocyte population. © 1992 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here