Premium
Differential expression of non‐major histocompatibility complex‐restricted cytotoxicity in patients with granular lymphocyte‐proliferative disorders associated with or unassociated with severe anemia
Author(s) -
Oshimi Kazuo,
Oshimi Yoko,
Motoji Toshiko,
Mizoguchi Hideaki
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830400204
Subject(s) - peripheral blood mononuclear cell , immunology , anemia , major histocompatibility complex , cd3 , bone marrow , lymphocyte , medicine , cd8 , biology , antigen , biochemistry , in vitro
Of 27 patients with granular lymphocyte‐proliferative disorders (GLPD), 18 patients had CD3 + T‐cell‐lineage GLPD (T‐GLPD), and 9 patients had CD3 − CD16 + natural killer (NK) cell‐lineage GLPD (NK‐GLPD). In 9 of the 18 patients with T‐GLPD, severe anemia of ≦7.5 g/dl hemoglobin (mean 5.4 g/dl) and erythroid hypoplasia in the bone marrow developed, while the remaining 9 patients with T‐GLPD and 9 patients with NK‐GLPD exhibited hemoglobin levels of ≧10.0 g/dl, and erythroid hypoplasia was not found. The number of leukocytes, neutrophils, lymphocytes or granular lymphocytes in the peripheral blood, or the percentage of lymphocytes in the bone marrow did not differ significantly between the patients with T‐GLPD associated with severe anemia and those with T‐GLPD not associated with severe anemia, and the immunophenotypes of peripheral blood mononuclear cells (PBMC) were not significantly different either. However, when non‐major histocom‐patibility complex (MHC)‐restricted cytotoxicity was assayed with PBMC, T‐GLPD patients with severe anemia and NK‐GLPD patients exhibited significantly higher levels of non‐MHC‐restricted cytotoxicity than T‐GLPD patients without severe anemia. Because PBMC obtained from T‐GLPD patients with severe anemia were shown not to lyse erythroblasts directly, the possibility that patient PBMC lyse erythroblasts in the bone marrow and thus cause anemia seems unlikely. The pathogenesis of anemia in GLPD was discussed. © 1992 Wiley‐Liss, Inc.