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DNA methylation patterns of the γδβ‐globin genes in human fetal and adult erythroid tissues
Author(s) -
Loo L. S. L.,
Cauchi M. N.
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830390410
Subject(s) - dna methylation , methylation , biology , microbiology and biotechnology , gene , erythroblast , epigenetics , globin , restriction enzyme , genetics , gene expression , stem cell , haematopoiesis
An investigation of the correlation between the γ→β‐globin switch and DNA methylation was carried out. The restriction patterns obtained with methylation‐sensitive and ‐insensitive enzymes indicated hypomethylation in the promoter region of the γ‐globin genes in fetal liver DNA but high methylation of the same region in all other samples (except in the presence of an elevated erythroblast count or leukemia). All samples appeared to be partially hypomethylated at the 5′ end of the δ‐globin gene and hypomethylated at the 3′ region of the β‐globin gene. Although consistent with a role for DNA methylation in globin gene regulation, the results also suggest that other factors besides methylation may be required for regulation of the level of expression, and switching of the globin genes. © 1992 Wiley‐Liss, Inc.