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Binding and suppressive activity of human recombinant ferritins on erythroid cells
Author(s) -
Fargion Silvia,
Cappellini Maria Domenica,
Fracanzani Anna Ludovica,
de Feo Tullia Maria,
Levi Sonia,
Arosio Paolo,
Fiorelli Gemino
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830390406
Subject(s) - ferritin , recombinant dna , k562 cells , cell culture , biology , binding site , chemistry , cell , microbiology and biotechnology , biochemistry , genetics , gene
We studied the relation between ferritin cellular binding and suppressive activity of recombinant H‐ and L‐ferritin on human erythroid cells at different proliferation/differentiation phases. L‐ferritin failed to show any suppressive activity or detectable binding to erythroblasts at any stage of maturation. In contrast, H‐ferritin demonstrated binding to erythroblasts derived from peripheral BFU‐E cells which increased steadily between 7‐14 days of culture up to 15,000 molecules per cell. Reticulocytes and erythrocytes failed to bind either L‐ or H‐ferritin. H‐ferritin suppressed BFU‐E colony formation and reduced K562 cell proliferation at nanomolar concentrations. This suggests that the expression of H‐ferritin binding sites is modulated by cellular proliferation and differentiation, that cells expressing H‐ferritin binding sites are sensitive to ferritin suppressive activity and that a causal relation exists between ferritin cellular binding and suppressive activity.