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Epidermal growth factor binding sites on human erythrocytes in donors with different ABO blood groups
Author(s) -
Engelmann Bernd,
Schumacher Udo,
Haen Ekkehard
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830390402
Subject(s) - abo blood group system , binding site , red blood cell , epidermal growth factor , dissociation constant , chemistry , microbiology and biotechnology , cooperative binding , blood cell , biology , biochemistry , immunology , receptor
Analysis of epidermal growth factor ( 125 J‐EGF) binding to human red cells revealed the presence of two classes of binding sites with apparent equilibrium dissociation constants (app.K d ) in the 10 −10 −10 −9 M and in the 10 −8 M range, respectively. The number of binding sites/cell ranged between 600 and 2,400 for the high‐affinity binding site and between 7,200 and 23,000 for the low‐affinity site. No differences were seen in the apparent K d values for both types of binding sites between red cells obtained from donors with different ABO‐blood groups. An increase in the number of high affinity EGF binding sites was observed in donors with blood group A,‐erythrocytes as compared to red cells taken from donors with blood groups o and B.