Premium
Immunological evaluation of harvested stem cells obtained by leukapheresis after chemotherapy
Author(s) -
Teixeira G.,
Lenormand B.,
Jean P.,
Fardoun D.,
SumereauDassin E.,
Bastit D.,
Tilly H.,
Piguet H.,
Monconduit M.,
Vannier J. P.
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830390304
Subject(s) - leukapheresis , immunophenotyping , cd34 , haematopoiesis , chemotherapy , progenitor cell , medicine , immunology , stem cell , flow cytometry , biology , genetics
Some patients suffering from malignancies may benefit of myeloablative chemotherapy followed by hematological reconstitution with autologous peripheral blood reinfusion. A quick evaluation of the number of hematopoietic progenitors present in leukapheresis blood samples is necessary to ensure the collection of a sufficient number of these cells. A study was performed on a series of 25 leukapheresis following initial chemotherapy. The number of granulomonocytic colony‐forming unit (CFU‐GM) and the number of CD34+ cells were evaluated simultaneously, in each sample. Results have shown a relatively strong linear correlation between both methods of evaluation of hematopoietic progenitors, suggesting that immunophenotyping could be a useful method to estimate the number of progenitors.