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CD45RA expression by CD4 T lymphocytes in tumors invaded by B‐cell non‐Hodgkin's lymphoma (NHL) or Hodgkin's disease (HD)
Author(s) -
Jacob MarieChristine,
Favre Mireille,
Lemarc'Hadour FrançOis,
Sotto MarieFrance,
Bonnefoix Thierry,
Sotto JeanJacques,
Bensa JeanClaude
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830390110
Subject(s) - lymphoma , cd5 , cd20 , cd19 , b cell , clone (java method) , monoclonal antibody , antibody , t cell , cd3 , immunology , medicine , immune system , cancer research , biology , pathology , cd8 , dna , genetics
Little is known about the role of tumor infiltrating T lymphocytes (TIL‐T) in the pathogenesis of malignant diseases and collaboration between normal and malignant cells has not yet been proved. In the present work, we have investigated whether immune T lymphocytes exist in tumors invaded by B‐cell non‐Hodgkin's lymphoma (NHL) or Hodgkin's disease (HD). Therefore, we have studied the reactivity of the CD45RA monoclonal antibody, which discriminates between naive and memory CD4 T lymphocytes. Our results showed far lower percentages of CD4+ CD45RA+ in malignant lymphoma (30.3 ± 15.0% in B‐cell NHL, and 37.4 ± 18.6% in HD) than in reactive hyperplasia (54.7 ± 13.2%), leading to the conclusion of an accumulation of immune cells in tumor microenvironment. A further heterogeneity in the relative proportion of naive and memory TIL‐T was also observed within lymphoma (range: 11 to 68% in B‐cell NHL, 5 to 69% in HD). In B‐cell NHL, it was related to histological features, as documented by the Kiel classification (P = .028), and to a stronger extent to cytological characteristics analysed with the Grenoble classification (P < .0001): class 1 NHL, which are essentially indolent NHL displayed lower naive cells (22.2 ± 7.4%) than class 3 NHL, which are more aggressive (40.1 ± 16.1%). Among the monoclonal antibodies (mAb) defining the B‐cell clone phenotype or activation state (CD19, CD20, CD21, CD22, CD23, CD24, CD5, CD10, CD11a, and Ki67), only CD23 (P = .0003) and Ki67 (p = .0007) revealed statistical association with the percentage of naive CD4 lymphocytes. No correlation could be demonstrated with the proportion of whole TIL‐T, activated CD3 DR TIL‐T, or CD4 subset.