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Physiologic and rheologic effects of the antisickling agent ethacrynic acid and its N‐butylated derivative on normal and sickle erythrocytes
Author(s) -
Orringer Eugene P.,
Blythe David S.,
Whitney James A.,
Brockenbrough Scott,
Abraham Donald J.
Publication year - 1992
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830390109
Subject(s) - hemoglobin , chemistry , red blood cell , diuretic , hemoglobin s , polymerization , derivative (finance) , cell , red cell , biochemistry , sickle cell anemia , pharmacology , biophysics , medicine , organic chemistry , biology , financial economics , economics , polymer
Ethacrynic acid, a loop diuretic, has been shown to inhibit hemoglobin S polymerization. Until now, however, most studies were performed using purified solutions of hemoglobin S. The experiments reported here were designed to examine the effects of ethacrynic acid and its n‐butryic acid derivative on the rheological and physiological properties of intact red blood cells. Using net and unidirectional flux measurements, both agents were shown to cause ion and water loss from normal and sickle erythrocytes. Since cell shrinkage adversely influences red cell rheology, it is unlikely that this class of compounds, despite its ability to inhibit hemoglobin S polymerization, will prove useful in the treatment of sickle cell disease.