Premium
Induction of carbonic anhydrase I isozyme precedes the globin synthesis during erythropoiesis in K562 cells
Author(s) -
Kondo Takahito,
Isobe Hiroshi,
Kawakami Yoshikazu,
Sakai Masaharu,
Nishi Shinzou,
Taniguchi Naoyuki
Publication year - 1991
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830380309
Subject(s) - hemin , carbonic anhydrase , isozyme , microbiology and biotechnology , incubation , k562 cells , carbonic anhydrase i , globin , biology , erythropoiesis , erythropoietin , gene isoform , biochemistry , enzyme , hemoglobin , heme , gene , anemia , endocrinology , medicine
Induction of carbonic anhydrase isozyme I (CA‐I) by erythropoietin or hemin was investigated using erythroleukemia (K562) cells. Immunological estimation and purification of carbonic anhydrases showed that untreated K562 cells contained only carbonic anhydrase isozyme II(CA‐II), while incubation of the cells with 2 units of erythropoietin (EP) per ml of the incubation medium or with 50 μM hemin resulted in the induction of CA‐I. The purified CA‐I induced in K562 cells was enzymatically and immunologically identical to that from mature erythrocytes. Flow cytometric analysis showed that incubation of K562 cells with EP as well as hemin induced CA‐I at the 3rd h, while α‐globin was detected at the 8th h. Northern blot analysis of CA‐I mRNA using a cloned genomic DNA as a probe showed that mRNA of CA‐I was induced by EP. These results suggest that induction of CA‐I is regulated at the transcriptional level during developmental changes of erythroid cells, and that CA‐I may play a physiologically important role during erythroid differentiation.