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Detection of immunoglobulin gene rearrangement in acute and chronic lymphoid leukemias of B‐cell lineage by polymerase chain reaction gene amplification
Author(s) -
Liang Raymond,
Chan Vivian,
Chan T. K.,
Wong Thomas,
Chiu Edmond,
Todd David
Publication year - 1991
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.2830380307
Subject(s) - polymerase chain reaction , immunoglobulin heavy chain , chronic lymphocytic leukemia , gene rearrangement , biology , antibody , leukemia , microbiology and biotechnology , acute lymphocytic leukemia , gene , b cell , lineage (genetic) , immunology , lymphoblastic leukemia , genetics
Polymerase chain reaction (PCR) was used to amplify the DNA fragments of the complementarity determining region 3 of the immunoglobulin (Ig) gene heavy chain from the leukemic cell specimens of patients with acute and chronic lymphoid leukemias of B‐cell lineage. Two different pairs of primers were tested. Fourteen of the 17 (82%) cases of acute lymphoblastic leukemia (ALL), and all 15 cases (100%) of B‐cell chronic lymphocytic leukemia, who had rearrangement of the Ig gene heavy chain by Southern analysis, were positive by PCR with either one or both pairs of primers. This technique was able to detect leukemic cells at the level of 0.1%. Applying it to study the remission marrow specimens following induction chemotherapy was more useful than morphology alone in predicting early relapse of the leukemia.